How to get rid of Comassie - (Nov/16/2005 )
I made up 0.5l of comassie stain yesterday. I let it stir overnight. So this morning I filtered it for the final product. Problem is, my stir bar is now blue. How do I get the stain off the stir bar? I have tried soap and water and an ethanol rinse. It is a teflon coated stir bar, and I managed to get a lot of it off, but the stain still wants to stick in spots.
Maybe if I put it in destain...
do you have alconox? that stuff is the bomb
I feel like a commercial here
but, for really tough lab stains and dishes, I make a solution in a dish tub with a little alconox and a small splash of bleach, add hot water and let the stir bar sit in it overnight
won't hurt the plastic, and should at least put a dent in your stain if it doesnt' get rid of it entirely.
I think perhaps you can try using the destaining solution for Commassie Blue. For example, methanol:glasial acid acetic at a ratio of 2:1. This is one of the destaining solution to get rid of the Commassie from PAGE gel.
Hope it helps.
I put it in commassie destain overnight. It got rid of most of it.