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PCR High GC content - PCR High GC content (Nov/16/2005 )

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Hi Shashi,

The best thing I've tried for high GC content (70%+) is using Betaine from Sigma, it is cheap and it is what most of the commercial additives are anyway (inc. Q solution). You can pay 100s of dollars for Q solution or about 10 for Betaine which does the same job.

Hope this helps



I use Pfu, DMSO, and a lot of MgSO4. my lovely intron won't work with anything else.


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