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spectrophotometry - blank pbs? (Nov/11/2005 )

can i blank with PBS (1x) for OD 280? the antibody is diluted in PBS.

-Orion-

QUOTE (Orion @ Nov 11 2005, 06:54 PM)
can i blank with PBS (1x) for OD 280? the antibody is diluted in PBS.


sure, you should always blank with the buffer your sample is diluted inside. why not?

-Kersten-