plasmids sequences - plasmids sequences (Nov/09/2005 )
I would REALLY appreciate any info concerning on how to get the sequences of pRP4 and pKM101.
I don´t know which vectors are those
but I can tell you what I ussually do.
I usually can get the sequence of the plasmids I currently use from PubMed.
Look up for nucleotide sequence type the name of the vector and search.
The good thing with having the sequence from PubMed is that you can dowload and import it to a program as vector NTI (you probably know it), and in your database you don´t get only the nucleotide sequence but also all the caracteristics of the vector, where you can easily see where is the promoter region, where is the MCS, antibiotic resistance gene, reporter gene, etc.
If you can not find it in PubMed, you just need to know the company that provided the plasmid (Promega, Stratagene, whatever...) and go to their online catalog as search for vector sequence.
You will find it for sure!!!