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resoltuion of SDS-PAGE - help (Oct/26/2005 )

Is it possible to separate two proteins of 35 and 36KDa in an 8% or 10%SDS-PAGE gel? what is the maximum resolution in the linear range of 8and 10%gels?



10% is not enough... 15% will do the jobsurely better.


To complete post of Fred I would also use quite a long gel and not a minigel cause separation is hard to differentiate 1kDA differences on a minigel

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