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help: - Doulbe strand or single strand for EMSA (Oct/17/2005 )

It was said that single strand of probe can also get good result for EMSA. What's the assumed principle under using the single strand or double strand?


think about the way a transcription factor works. I think you will get best results with a double-stranded probe, because you are mimicking the promoter region where the TF binds, right?

I label just one strand and then anneal the second (unlabelled) strand

I have not tried it with single-stranded DNA but I bet you would not see good binding efficiency.