change between neomycin and blasticidin resistance gene - (Oct/17/2005 )
What Fred is saying is that the overhanging sequence is the same but since the bases on either side of the overhang are different, ligating will not regenerate a restricton site. We've used this before, quite handy trick. Say Fred, I am going back to Paris in a few months for x-mas. This isn't biology related but can you suggest any good hotels that won't cost an arm and a leg and isn't far from the center of town?