Protocol Online logo
Top : Forum Archives: : Molecular Biology

How do I extract a HUGE plasmid? - (Feb/01/2001 )

I need to extract an 80-90 kb plasmid from E. coli. I can't find any protocols to accomlish this. Any ideas?

--

What is the copy number of the plasmid? Why can't you use a Qiagen Kit? How much DNA do you require?

Best regards,

Andrew

--