90% confluency - (Aug/05/2009 )
bob1 on Aug 6 2009, 05:24 PM said:
The problem with confluency is that it is different for different cell lines. I occasionally work with brain cell derived cells that have very long processes and are contact inhibited, so while only 30-40% of the apparent flask area is covered, the cells stop growing having reached 100% confluency by the processes touching.
In terms of area, your cells will be about 50-70%, though the picture is a little unclear and without knowing the cell line it is hard to judge how confluent they actually are.
In terms of area, your cells will be about 50-70%, though the picture is a little unclear and without knowing the cell line it is hard to judge how confluent they actually are.
Thanks to all of you for sharing your idea. this is one patient's (Human) skin fibroblast what we have grown at lab.
-epigenetics-
Hard to tell the confluency, I wouldn't say it's 90% though.
I think you need to determine it experimentally - see how dense they will grow and once they have reached that point you know what 100% looks like. Then just go a little less I guess.
-Stephan-
epigenetics on Aug 5 2009, 09:01 PM said:
Hello,
We decided to go till 90% conflency level of human skin fibroblast cells. and theres a problem, the pic one of my lab members showed me for 90% conflency, other one argued that it is 60-70%. My cells are getting ready for harvesting, Experts, would you be able to show me any pic of cells at 90% confluency.
so that i can do it properly,
Thanks,
We decided to go till 90% conflency level of human skin fibroblast cells. and theres a problem, the pic one of my lab members showed me for 90% conflency, other one argued that it is 60-70%. My cells are getting ready for harvesting, Experts, would you be able to show me any pic of cells at 90% confluency.
so that i can do it properly,
Thanks,
The only true way of estimating the confluency is to use a Graticule and count the number of cells in a field. If you grow cells to what you think is 100% and count with a graticule, then subsequent measures of confluency are easy.
Of course every cell/line is different (size and shape), so you would have to do this process with every cell you use.
Kindest regards
Rhombus
-rhombus-