Protocol Online logo
Top : New Forum Archives (2009-): : ELISA and Immunoassay

ELISA Help-stopping - stopping with HCL vs H2SO4 (Jul/20/2009 )

Is it possible to stop an ELISA (HRP used as enzyme, OPD/H2O2 as the substrate) with concentrated HCL? I see many protocols calling for stopping with H2SO4, will there be any difference in OD values since both HCL and H2SO4 will make the solution very acidic?

Thanks!

-tholusc-

Yes you can, I've used both in different elisa's en didn't notice a difference.
Don't use concentrated HCl but 1 or 2 M HCL

-Gerard-