olligo annealing for shRNA cloning - (May/26/2009 )

Hi Enthusiast,

In the protocol you mentioned above,

5ul Forward oligo
5ul Reverse oligo
5ul 10x NEB buffer2
35ul ddH2O

Is the "10X NEB buffer2" same as the reaction buffer they provide with restriction enzyme?

I have the exactly same problem now, and in my case, I am using 10x annealing buffer from Gendepot.

After several times of failure, I am not a fan of their annealing buffer. I am up to follow new protocol.