dominat negative mutants - (Feb/16/2009 )

Hi,

What are dominant negative mutants? E.g. read an article where they transfeted cell lines with dominant negative form of Rac1?

Thanks

I think it means that the line has a dominant mutation of a loss of function.

If you give the reference of the article we might be more helpful.

from wikipedia:

Dominant negative mutations (also called antimorphic mutations) have an altered gene product that acts antagonistically to the wild-type allele. These mutations usually result in an altered molecular function (often inactive) and are characterised by a dominant or semi-dominant phenotype. In humans, Marfan syndrome is an example of a dominant negative mutation occurring in an autosomal dominant disease. In this condition, the defective glycoprotein product of the fibrillin gene (FBN1) antagonizes the product of the normal allele.

molgen on Feb 16 2009, 06:25 PM said: