% INPUT - ChIP - (Feb/05/2009 )

Hello everyone,

I have read those comments and find it very useful. Thank you very much.

I got some problems in analyzing my results and might need your help. In my case. I took 10ul of sonicated chromatin for input and 100ul for ChIP. After DNA extraction, I used 1 ul DNA for realtime PCR. I didn't measure the DNA concentration of any sample. How does it happen when GAPDH was undetectable while my target gene was detectable in mock and IP samples?

Another one, I used plasmids at different number of copies to make standard curve. So is there any notice when analyzing realtime PCR result?

Looking forward to your reply.