% INPUT - ChIP - (Feb/05/2009 )
Thanks for explaining the calculation so well. I have a naive question about error bar calculation. I know how to determine the % input of ChIP'd DNA but I am not sure how to calculate errors for plotting error bars in my graph. I just got a stardard deviation from my triplicate PCR reaction, then how to convert it into a positive and a negative error.
M&M81 on Feb 27 2009, 01:29 AM said:
Do you guys calculate the %input of the triplicate PCR individually and then get a SD from them? so the error bar is equal to the SD calculated?
M&M81 on Feb 26 2009, 05:39 PM said:
M&M81 on Feb 27 2009, 01:29 AM said:
Do you guys calculate the %input of the triplicate PCR individually and then get a SD from them? so the error bar is equal to the SD calculated?
you can either use SD for error bar, or you can use SEM, which is equal to SD / square root
for people working on animals, usually the variation is bigger and SEM would make more sense
Hi jiro_killua,
I used your method to calculate % of input for my samples. The result I got was the protein i studies do not bind to promoter region after treatment!
But when I carried out normal PCR, I can see a much more intense band on my agarose gel after treatment. Can you tell me why?
I really dont know how to study all the figure I got from qPCR!