non- linear Scatchard plot using radio isotope labeled ligand. (View forum version)



RNA woman

Posted 18 May 2016 - 12:22 AM

How to calculate non- linear Scatchard plot using adhered cell culture and radio isotope labeled ligand. I am studying the cooperativity in EGFR .

I am asking for the most straight forward/ easy to use method. please guide me to tutorial or any other source that explain this in details. 


mdfenko

Posted 18 May 2016 - 03:40 AM

the scatchard plot should always be linear. if it's not then the ligand binding has not reached equilibrium.

 

however, it could exhibit multiple linearity if a threshold is reached where the binding protein undergoes a conformational change which causes the binding affinity to change or opens up secondary binding sites.