Problems of beta-actin (View forum version)

cell 293

Posted 11 September 2014 - 08:13 PM

Hi, everyone


This is beta-actin. I got multi-signals about this. I am sure i didn't move the film when it was exposed to signal.




Was too much sample loaded? 


The same samples were used many times. It is no problem when i use it first time.


Was the store method wrong? I store sample in -20°C.



Thanks a lot.



Posted 13 September 2014 - 01:48 PM

Non-spcific antibody reactions, use a shorter secondary incubation (1h or less) and try using a higher dilution of primary.