fixation and methylation (View forum version)



neuron00

Posted 06 April 2014 - 10:59 AM

I am planning on extracting DNA from tissues for a methylation study.  Would a prior methanol fixation affect the DNA extraction or methylation?  Thanks


phage434

Posted 06 April 2014 - 06:43 PM

I doubt if this will be a problem.


pcrman

Posted 08 April 2014 - 09:03 AM

Nope. But keep in mind, since DNA from paraffin blocks is already degraded, and will be further fragmented during bisulfite conversion, amplicons size should be kept to a minimum when designing primers.