Problem in Cell Viewing (View forum version)



Celz

Posted 06 February 2014 - 12:40 AM

Dear all, 

 

I was using Anthony's staining method to view the encapsulated bacterial cells. I have a question that would like to seek for your advice. Attached is the cells image after staining with crystal violet. 

 

First image (IMG_6985) is the bacterial cells incubated with maltodextrin and fructo-oligosaccharide. Did anyone know what is that pinkish stuff? Is maltodextrin or fructo-oligosaccharide? Or the combination of both of them?

 

Second image (IMG_7007) is the bacterial cells incubated with maltodextrin only. I noticed there is a number of sphere shape capsule-like compound. Is that the encapsulated bacterial cells? 

 

Thank you. 

  • IMG_6985.JPG
  • IMG_7007.JPG

El Crazy Xabi

Posted 06 February 2014 - 03:55 PM

Magnification? Is bright field microscopy?

The second looks more like bubble-like stuff but no cells.


Celz

Posted 06 February 2014 - 04:07 PM

Magnification is 100x, under light microscope. Yup. I was suspecting the bubble like stuff is actually maltodextrin, do you think there are cells being encapsulated inside the bubble-like stuff which not able to stained out using crystal violet? Thanks. 


El Crazy Xabi

Posted 06 February 2014 - 06:13 PM

Hard to say without a reference of a naked cell