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AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (Jan/27/2014 )

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Thanks a lot. If possible, can you share a link of your publication please?

-Nilufer-

This one.

β-Thalassemia Due to Intronic LINE-1 Insertion in the β-Globin Gene (HBB): Molecular Mechanisms Underlying Reduced Transcript Levels of the β-GlobinL1 Allele

 

It's only a brief report though, so there wasn't much space to discuss it, but it cites this paper, that originally showed that AZA is not the cause of increased expression of gamma-globin (which is silenced by hypermethylation in adults):

 

Mabaera R, Greene MR, Richardson CA, Conine SJ, Kozul CD, Lowrey CH. 2008. Neither DNA hypomethylation nor changes in the kinetics of erythroid differentiation explain 5-azacytidine’s ability to induce human fetal hemoglobin. Blood
111:411–420.

-Trof-
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