Cytokines feeding cell culture (View forum version)



yyc

Posted 14 September 2013 - 12:24 PM

Hi everybody,

I need to add cytokines to my cell cultures and in some protocols say I should add certain amount of cytokines per number of cells and in other protocols only refer to cytokines concentration in the culture.

If I consider cytokine concentration it looks it is irrelevant the cell number I have in the culture. But maybe for stimulation with cytokines it is in fact important the amount of cytokines per number of cells.

I would appreciate your comments! thanks!
 

Tarique

Posted 15 September 2013 - 04:11 PM

Usually no of cells seeded per well depend on the surface area of the well; I ue 1e5 per cm2; the vol of media depends on the duration of the culture; finally, amount of cytokine usually depends on the vol of media added.