Advice on sonication (c2c12) - (Apr/19/2012 )
Thanks KPDE, will give that a shot.
The lysis buffers in that document are already the same ones that I am using, memari.
Base on my experience ,1% SDS is nessesary (in nuclear lysis buffer) to break nucleus and DNA.
And you should use a nuclear fraction to do it.
Use Cytoplasmic Lysis buffer to break the cell and then use nuclear fraction with a nuclear lysis buffer that contain 1% SDS.
That's exactly what I'm doing