Protocol Online logo
Top : New Forum Archives (2009-): : ChIP and Next Generation Sequencing

Advice on sonication (c2c12) - (Apr/19/2012 )

Pages: Previous 1 2 

Thanks KPDE, will give that a shot.

The lysis buffers in that document are already the same ones that I am using, memari.

-Sel-

Base on my experience ,1% SDS is nessesary (in nuclear lysis buffer) to break nucleus and DNA.
And you should use a nuclear fraction to do it.
Use Cytoplasmic Lysis buffer to break the cell and then use nuclear fraction with a nuclear lysis buffer that contain 1% SDS.

-----
Babak Memari

-memari-

That's exactly what I'm doing

-Sel-
Pages: Previous 1 2