Basis/Protocols for Choosing concentrations in testing medicinal effect plant ex - (Jan/31/2012 )
I was conducting a study to test a medicinal effect of a specific plant..I did not see any protocols on what concentration should i used. I decided to use 25%,50%,75% and 100% of the extract but still i should have a basis for using it..Anyone can help me on this...thank you so much..
I guess you would like to use a compound at a concentration comparable to its affinity or better to its EC50 to its intended target protein.
it is really depend on the method of extraction is it a simple infusion or a super critical extraction method? bellow you could find a useful guide: