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How to store sample organism for future use - (Sep/16/2010 )

I would like to preserve my mosquitoes for a few months before i extract DNA/RNA from them? Can i keep them in 60% ethanol?

-hianghao-

hianghao on Fri Sep 17 05:18:14 2010 said:


I would like to preserve my mosquitoes for a few months before i extract DNA/RNA from them? Can i keep them in 60% ethanol?

It is one possibility, though I used 70% for insects....anyway best is normally to freeze them directly without any preservative. And be sure that, if you use denatured ethanol, the other ingredients don't interfere with your mosquitoes/DNA...some of these denaturing agents seem to counteract DNA isolation or degrade DNA...

-hobglobin-

use: http://www.ambion.com/techlib/prot/bp_7020.pdf

...or snap-freeze in liquid nitrogen and store at -80C should work!

-moljul-