How to store sample organism for future use (View forum version)



hianghao

Posted 16 September 2010 - 09:18 PM

I would like to preserve my mosquitoes for a few months before i extract DNA/RNA from them? Can i keep them in 60% ethanol?

hobglobin

Posted 17 September 2010 - 11:23 AM

I would like to preserve my mosquitoes for a few months before i extract DNA/RNA from them? Can i keep them in 60% ethanol?

It is one possibility, though I used 70% for insects....anyway best is normally to freeze them directly without any preservative. And be sure that, if you use denatured ethanol, the other ingredients don't interfere with your mosquitoes/DNA...some of these denaturing agents seem to counteract DNA isolation or degrade DNA...

moljul

Posted 20 September 2010 - 04:16 AM

use: http://www.ambion.co...rot/bp_7020.pdf

...or snap-freeze in liquid nitrogen and store at -80C should work!