protein concentration per well (View forum version)


Posted 27 July 2010 - 03:23 AM

Hello Scientists,
some one told me that protein is loaded with respect to the size of the wel, let say 10 micrograms per one cm, is it true or not. What i assume was 10 micrograms per micro liter.
hope to get answer soon



Posted 27 July 2010 - 03:33 AM

i have never heard of any such concept... u load watever amount u want in each well depending on ur application taking care u dont under or overload the protein!!!
what amount u can load depends on the protein concentration and the well dimention.. tats it!!!


Posted 27 July 2010 - 09:36 PM

Thanks...may u b blessed wid great achievements in life.


Posted 27 July 2010 - 10:29 PM

:lol: :lol: :lol:

thanx dude!!!


Posted 27 July 2010 - 10:40 PM

In continution with SDS PAGE, can we reuse the electrode buffer which is incontact with the samples in stacking gel.



Posted 27 July 2010 - 11:01 PM

well if u are a very poor loab then u may reuse it.. but i wud not reuse the running buffers!!! :D


Posted 28 July 2010 - 01:08 PM

Protein and DNA/RNA are quite frequently loaded based on the width of the lane, if you load too much you get a blob with some smearing for a band, if you load too little, you won't be able to see the band. The quantities people usually say to load are typically for 8 mm lanes.

You can reuse buffer about once, but it won't work very well as the electrolytes run out.