Methanol vs Isopropanol in fixing solution (View forum version)



polaris

Posted 10 June 2010 - 08:46 AM

General question: What is the difference between using methanol/acetic acid versus isopropanol/acetic acid when fixing proteins in gels?

mdfenko

Posted 23 June 2010 - 10:36 AM

functionally, no difference.

concentration of alcohol necessary is different (i think you need 10% isopropanol as opposed to 30-50% methanol).