Protein A-agarose or A-sepharose for immunoprecipitation? (View forum version)



jasmina

Posted 30 April 2010 - 01:48 AM

Hi all,
I used dynabeads protein G for with anti-rabbit antibody to immunopurify my protein. but, i couldnt' immunoprecipitate!
I'm wondering if its question of protein G, and i want to try Protein A. I found protein A-agarose aliqot and i want to try to check if i could pull down!
i would like to ask if protein A-sepharose is better than A- agarose in my case (to purify my protein from cerebral lysate)!!
thanks for help

mdfenko

Posted 30 April 2010 - 06:55 AM

sepharose is ge healthcare's (nee pharmacia) brand name for agarose. protein a-sepharose and protein a-agarose are virtually the same.

jasmina

Posted 30 April 2010 - 07:02 AM

ah ok,!! i see!
because one collegue that A-sepharose is more used and better than agarose!
thanks for your answer!

mdfenko

Posted 30 April 2010 - 07:07 AM

well, there are some aging and quality control issues but for your purpose it should make no significant difference (i prefer sepharose to generic agarose).