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How Best to Clean the Sonicator Tip Between Samples? - (Oct/28/2009 )

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! :)

-Envennom-

What about disposable probe covers? They make them for thermometers, but maybe they will work for sonicators.

http://www.fishersci.com/wps/portal/ITEMDE...uctsItemsFlag=Y

http://www.labsafety.com/Welch-Allyn-SureT...160/?CID=9PP001

http://www.allegromedical.com/diagnostic-p...11124bc743356ff

-lab rat-

I clean it between samples with the same buffer i am using to sonicate. And after that with ethanol

-laurequillo-

Envennom on Oct 28 2009, 11:43 PM said:

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! ;)



i just wipe the tip with 70% EtOH.

-DRN-

Envennom on Oct 28 2009, 11:13 AM said:

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! :rolleyes:


Envennom,

wiping with ethanol or sonication of ethanol in a beaker is an effective way.

Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.

Save time and focus on your research instead of performing tedious cleaning tasks :P

-Hielscher_Ultrasonics-

Hielscher_Ultrasonics on Nov 23 2009, 08:56 AM said:

Envennom on Oct 28 2009, 11:13 AM said:

Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! ;)


Envennom,

wiping with ethanol or sonication of ethanol in a beaker is an effective way.

Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.

Save time and focus on your research instead of performing tedious cleaning tasks :)

Would cleaning with autoclaved water be sufficient to avoid cross contamination?

-Julie123-