Is Taq polymerase still active after staying at 10C for one day? (View forum version)



DigitalPig

Posted 24 October 2009 - 06:49 PM

I accidentally left the polymerase tube outside the fridge for one day, (temp is around 10C since it previously in the ice bath...). Can I still use this Taq polymerase? Thanks!

perneseblue

Posted 24 October 2009 - 06:56 PM

I accidentally left the polymerase tube outside the fridge for one day, (temp is around 10C since it previously in the ice bath...). Can I still use this Taq polymerase? Thanks!


Yes. Give it a try. It should be okay from this miss adventure.

phage434

Posted 24 October 2009 - 06:56 PM

Yes, almost certainly. Taq is a high temperature enzyme which survives 95C in a thermocycler. The main danger would be bacterial or fungal growth, but this is unlikely over a day, and probably would be inhibited by other components of the enzyme buffer.