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Knockdown Related Discussions
  1. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
  2. Knockdown with shRNA not stable (Why?) - (reply: 3)
  3. miRNA knockdown in a mouse, tail vein injection (~50% KD) - (reply: 2)
  4. Antibody validation for ChIP - high backgound after knockdown - (reply: 4)
  5. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
  6. Gene knockdown expression levels shRNAmirs - (reply: 2)
  7. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
  8. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
  9. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
  10. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
  11. Little knockdown but big effect on proliferation - (reply: 2)
  12. Knockdown induce apoptosis - (reply: 5)
  13. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
  14. Confirmed gene knockdown not showing on array - (reply: 2)
  15. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)
  16. miRNA knockdown experiment controls - Very confused (reply: 2)
  17. miRNA knockdown approaches - (reply: 6)
  18. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
  19. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  20. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
  21. Help: protocols for shRNA knockdown effect on Ag-specific Treg differentiation - (reply: 1)
  22. nonreproducible knockdown efficiency with Plemir-micorRNA from Open Biosystem - (reply: 3)
  23. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  24. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
  25. Dicer knockdown - (reply: 6)
  26. How to do stable knock-down by shRNA advices for beginner - (reply: 1)
  27. Effect of MOI on lentiviral shRNA knockdown - (reply: 1)
  28. Why there is no knockdown? - (reply: 3)
  29. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  30. Lentivial Knockdown of miRNAs - (reply: 8)
  31. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  32. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  33. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
  34. Knockdown of AcpP in S. enterica while in macrophages - PubMed link (reply: 1)
  35. stable transfection problems - stable knockdown (reply: 3)
  36. Knockdown of miRNAs - (reply: 8)
  37. RNAi in Zebrafish - vector for zebrafish knock-down (reply: 4)