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  • Culturing HEK 293 Cells (Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
    http://www.pharma.ethz.ch/institute_groups/biomacr...
    Added: Mon Sep 09 2002, Hits: 1881, Reviews: 2 Read reviews  Write review   Cached

    • 4 of 37 people found the following review helpful:
    Wake up HEK 293 cells, Wed Jun 25 2003
    Reviewed By: Maruf Ahmed
    Cell stock wake up

    MATERIALS
    15ml conical bottom tube
    35-mm culture dishes

    Culture Media
    Dulbeccos Modified Eagles Medium (DMEM)
    (medium is fortified with 10% FBS and antibiotic)

    METHODS

    Cell stock tubes from liquid nitrogen store. Melt in water bath, take out when still some ice left. Take 15ml conical bottom tubes. Add 8ml culture medium into tubes. Put melted cell suspension into tubes. Centrifuge at 1000rpm, 5min.
    Aspirate medium. Add 1ml culture medium
    Mix by pipeting and gentle shaking. Take a 35mm collagen coated dish (HEK). Put 3ml (35-mm dish) culture medium into plates. Add cell suspension dropwise. Incubate at 37°C.

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    7 of 33 people found the following review helpful:
    HEK 293 cell, Wed Jan 15 2003
    Reviewed By: de Silva
    Reviving of the 293 cells are not elicited properly, it would be great if it had more details

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