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1. Raising monoclonal antibodies - Monoclonal antibodies raised against similar sequence Antigens in mice (reply: 2)
3. staining mitotic spindle of leukocytes - a question from a total novice (reply: 3)
5. secondary Ab for immunofluorescent staining - secondary Ab designed for WB was used for IHC (reply: 5)
7. What's the major cell type in thymus - (reply: 1)
9. immunofluorescence of parafin embedded tumor section - (reply: 2)
11. What kind of cells is it? - need which counterstain? (reply: 1)
13. About antigen-antibody affinity - (reply: 3)
15. titrations - (reply: 3)
17. heat retrieval - necessary or not? - his-tagged protein detection (reply: 3)
19. Difference between PCDNA and Brdu - (reply: 1)
21. Signal in the citoplasm instead of in the nucleus - (reply: 1)
23. ELISA versus Neutralizing Titer - (reply: 4)
25. Griess Assay reagents - Expiry date? (reply: 2)
27. So what is fluorescence SUPPOSED to look like? - fluorescent secondary antibody scans as SOLID BLACK image (reply: 1)
29. antibody against His-tagged protein - (reply: 3)
3. staining mitotic spindle of leukocytes - a question from a total novice (reply: 3)
5. secondary Ab for immunofluorescent staining - secondary Ab designed for WB was used for IHC (reply: 5)
7. What's the major cell type in thymus - (reply: 1)
9. immunofluorescence of parafin embedded tumor section - (reply: 2)
11. What kind of cells is it? - need which counterstain? (reply: 1)
13. About antigen-antibody affinity - (reply: 3)
15. titrations - (reply: 3)
17. heat retrieval - necessary or not? - his-tagged protein detection (reply: 3)
19. Difference between PCDNA and Brdu - (reply: 1)
21. Signal in the citoplasm instead of in the nucleus - (reply: 1)
23. ELISA versus Neutralizing Titer - (reply: 4)
25. Griess Assay reagents - Expiry date? (reply: 2)
27. So what is fluorescence SUPPOSED to look like? - fluorescent secondary antibody scans as SOLID BLACK image (reply: 1)
29. antibody against His-tagged protein - (reply: 3)