Summary: Real-Time PCR primers sets may be designed using standard primer design algorithms without any modification, except that the amplicon size is smaller. As with all PCR amplifications, however, the specific reaction conditions for each set must be optimized, particularly primer concentration, annealing temperature and magnesium chloride concentration.
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Protocols
- Real-Time PCR (qPCR) Primer Design Tool (IDT)
You can input a sequence accession number or a sequence, the program will return qPCR primers.
http://biotools.idtdna.com/Scitools/Applications/R...
Added: Thu Feb 05 2009, Hits: 11849, Reviews: 0 Write review Cached -
Real-time PCR (TaqMan) Primer Design (GenScript)
This online tool designs real-time PCR (TaqMan) primers for you. It accepts an accession number or a sequence as input
https://www.genscript.com/ssl-bin/app/primer
Added: Thu Feb 05 2009, Hits: 5733, Reviews: 0 Write review -
Primer Bank
(Dr. Xiaowei Wang at the Department of Molecular Biology, Massachusetts General Hospital)
PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). There are several ways to search for primers: by GenBank Accession, NCBI protein accession, LocusLink ID, PrimerBank ID or Keyword (gene description). PrimerBank contains about 180,000 primers covering most known human and mouse genes.
http://pga.mgh.harvard.edu/primerbank/index.html
Added: Fri May 14 2004, Hits: 5086, Reviews: 0 Write review Cached -
Real-Timer PCR Primer Database
(Steven W. Johnson, Ph.D.)
A frequently updated database of validated primer/probe sets for quantitation of a large number of genes of various organisams.
http://www.realtimeprimers.org/
Added: Sat Mar 27 2004, Hits: 7732, Reviews: 0 Write review Cached