Top : Research Tools : Online Tools : PCR Primer and Oligo Design : Real-Time PCR Primer Design Tools
Summary: Real-Time PCR primers sets may be designed using standard primer design algorithms without any modification, except that the amplicon size is smaller. As with all PCR amplifications, however, the specific reaction conditions for each set must be optimized, particularly primer concentration, annealing temperature and magnesium chloride concentration.
Related Categories:


Sponsored Links