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Top : General Laboratory Techniques : Water Treatment with DEPC : Water Treatment with DEPC
Water Treatment with DEPC | |
| Author: Protocol Online | |
| Source: Protocol Online | |
| Date Added: Thu May 08 2003 | |
| Date Modified: Tue Feb 17 2009 | |
| Abstract: Treat water with DEPC to eliminate RNase | |
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave 20 min to destroy DEPC by causing hydrolysis of DEPC.
Notes:
DEPC is highly toxic.