Estimation of Fat in Milk Samples
|Author: * Sanjeev Sharma1, BR Yadav1, Aarti Bhardwaj2, Sha|
|Affiliation: 1 Livestock Genome Analysis Lab, 3 Animal Biochemistry Division, National Dairy Research Institute, Karnal- 132001, India. 2 Meerut Institute of Engeenering and Technology, Meerut, U.P., India|
|Source: Protocol Online|
|Date Added: Mon Feb 02 2009|
|Date Modified: Mon Feb 02 2009|
|Abstract: This protocol is a simple and explained with brief introduction and principle. This is highly useful for the researchers working in the field of dairy.|
Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessary to know for casein/fat ratio normalization. It is also important for the dairyman to know milk fat content exactly: discrepancies in the results of milk fat tests (usually performed in the dairy industrial plant) have economic relevance. In addition, a low milk fat content could indicate the existence of animal health deficiencies.
The fat can be separated from fat-containing milk/milk powder
through the addition of sulphuric acid. The separation is made by using amyl
alcohol and centrifugation. The fat content is read directly on a special
calibrated butyrometer after centrifugation.
Sulphuric acid is toxic, highly corrosive and will cause
severe burning if it comes in contact with the skin or eyes.
When mixing the butyrometer contents, considerable heat is generated.
If the stopper is slightly loose, leakage may occur during mixing, centrifuging or holding in the water bath.
Wear protective eye goggles
Avoid all spillage and dropping of sulphuric acid from acid dispensers.
When mixing, hold the butyrometer stopper firmly to ensure that it cannot slip. Use a cloth or glove to protect the hands when mixing.
Do not point the butyrometer at anyone when mixing.
* Sanjeev Sharma1, BR Yadav1, Aarti Bhardwaj2, Shalini Jain3 and Hariom Yadav3
1Livestock Genome Analysis Lab, 3Animal Biochemistry Division, National Dairy Research Institute, Karnal-
2Meerut Institute of Engeenering and Technology, Meerut, U.P., India