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• Cell Biology/Cell Culture/Neural Cell Culture

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Protocols

• neuromelanin isolation and identification (Sulzer lab, Depts. Neurology & Psychiatry, Columbia University)
  Details on EPR for neuromelanin detection
  http://www.columbia.edu/~ds43/EPR.html
  Added: Tue Aug 13 2002, Hits: 165, Reviews: 0  Write review   Cached
• Preparation of Brain Membrane Fractions for Western Blot Analysis (BD PharMingen)
  This is a standard protocol adapted from Rogers et al., 1991.
  http://www.bdbiosciences.com/pharmingen/protocols/...
  Added: Tue May 14 2002, Hits: 2171, Reviews: 0  Write review   Cached
• Primary Neuron Viability Assay (The Science Advisory Board)
  luorescence based asssay for establishing neuronal viability in cell culture monolayers.
  http://www.scienceboard.net/resources/protocols.as...
  Added: Thu Feb 19 2009, Hits: 677, Reviews: 0  Write review   Cached
• Protocol for Labeling of Neurons cells - immunohistochemistry (Fatih Kocabas, Moleküler Biyolog ve Genetikçi, UT Southwestern Medical Center at Dallas)
  http://fatihkocabas.net/neuronlabelling.aspx
  Added: Sat Jan 31 2009, Hits: 822, Reviews: 0  Write review   Cached
• Protocol for the Primary Culture of Cortical and Hippocampal neurons (Steven Finkbeiner, UCSF)
  This protocol describes the following: Solutions and media required / Set-up for the dissection /Dissection of hippocampus and cortex / Papain treatment /Trypsin Inhibitor treatment /Trituration and Plating cells.
  http://www.gladstone.ucsf.edu/gladstone/files/fink...
  Added: Tue Oct 01 2002, Hits: 2094, Reviews: 0  Write review   Cached
• Studying the Neural Basis of Adaptive Locomotor Behavior in Insects (JoVE)
  This protocol uses a slippery surface setup to investigate the timing of leg muscles in the behaving stick insect with respect to touch-down and lift-off under different behavioral paradigms such as straight forward and curved walking in intact and reduced preparations.
  http://www.jove.com/Details.php?ID=2629
  Added: Thu Apr 14 2011, Hits: 94, Reviews: 0  Write review