Top : Molecular Biology : RNA : microRNA : microRNA Microarray

Summary: microRNA microarray is a high throughput method for studying microRNA expression in cultured cells or tissues. Compared to traditional cDNA microarry expression profiling, RNA samples used for microRNA microarray hybridization are required to be enriched for small RNAs which are of 20-30-nt in size. A microRNA microarray experiment generally consists of the following steps: 1) Total RNA isolation and small RNA enrichment or direct small RNA isolation; 2) microRNA labeling and clean-up; 3) microRNA hybridization to arrays spotted with microRNA probes; 4) Washes and scanning.
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