Summary: microRNA microarray is a high throughput method for studying microRNA expression in cultured cells or tissues. Compared to traditional cDNA microarry expression profiling, RNA samples used for microRNA microarray hybridization are required to be enriched for small RNAs which are of 20-30-nt in size. A microRNA microarray experiment generally consists of the following steps: 1) Total RNA isolation and small RNA enrichment or direct small RNA isolation; 2) microRNA labeling and clean-up; 3) microRNA hybridization to arrays spotted with microRNA probes; 4) Washes and scanning.
- microRNA Microarray Protocol (Microarray Core Facility, UT Southwestern Medical Center at Dallas )
This protocol consists of three major parts of detailed procedures for microRNA microarray experiment including microRNA isolation and purification, miRNA labeling and clean-up, and microRNA array hybridization, washing and scanning.
Added: Sat Jan 31 2009, Hits: 2400, Reviews: 0
This product manual may give you a general idea and guidance how microRNA microarray experiments are performed.
Added: Sat Jan 31 2009, Hits: 2266, Reviews: 0
(McManus Lab, UCSF)
This protocol covers the following steps: 1. Post-Processing (Can be done ahead of time), 2. Isolation of Small RNAs from sample (Can be done ahead of time), 3. Labeling of RNA (Done the day of hybridization), 4. Hybridization, 5. Washing and Scanning (Done the day after hybridization)
Added: Sat Jan 31 2009, Hits: 1504, Reviews: 0