Protocols
- S1 Nuclease Analysis of mRNA Levels
(The Flemington Lab, Tulane Health Sciences Center)
http://www.flemingtonlab.com/Protocols/S1NucleaseP...
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Ribonuclease Protection Assay
(Frank Bottone, Jr.)
http://www.unc.edu/~fbottone/protocol/rpa.html
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RNase Protection Assay
(BD PharMingen)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. This is a standard protocol.
http://www.bdbiosciences.com/pharmingen/protocols/...
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RNase Protection Assay
(NWFSC)
Most RNase Protection protocols require an overnight hyb with numerous subsequent clean-up steps. This method requires a maximum hyb of four hours, and the clean-up steps are the barest minimum, yet still produce nice images
http://micro.nwfsc.noaa.gov/protocols/RNAseProtect...
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RNase Protection Assay
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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RNase Protection Assay
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
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RNase Protection Assay
(Beverly Faulkner-Jones)
This method can be used to detect and quantitate mRNA, to map mRNA termini and to determine the position of introns within the corresponding gene.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
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S1 mapping with of yeast RNA with oligonucleotide probe
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://www.fhcrc.org/labs/hahn/methods/mol_bio_met...
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S1 Nuclease protection
(Gottschling Lab, Fred Hutchinson Cancer Research Center)
probe preparation, S1 mapping
http://www.fhcrc.org/science/labs/gottschling/misc...
Added: Fri Jul 26 2002, Hits: 2151, Reviews: 0 Write review Cached