Protocols
- Performing RNAi Experiments in Animals (Ambion)
An introduction to RNAi in vivo. There are two basic methods for triggering RNAi have been adapted for use in vivo: delivery of siRNAs, and delivery of plasmid and viral vectors that express a short hairpin RNA (shRNA) that is subsequently processed into active siRNA.
http://www.ambion.com/techlib/tn/131/5.html
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in vivo RNAi Protocols
(Invitrogen)
Covering many aspects of RNAi in vivo applications
http://www.invitrogen.com/site/us/en/home/Products...
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Producing siRNA for in vivo applications
(BioTechniques)
SiRNA is widely used for analyzing gene function and target validation in vitro and in recent years has been successfully extended to in vivo research. Integrated DNA Technologies (IDT) synthesizes siRNAs for both in vitro and in vivo applications. Researchers planning in vivo work should be aware that potentially toxic substances can be introduced during oligonucleotide synthesis and purification (HPLC) processes, which must be removed to prevent erroneous results and/or the death of laboratory animals.
http://www.biotechniques.com/default.asp?page=prot...
Added: Sat Jan 31 2009, Hits: 1158, Reviews: 0 Write review Cached