Subcategories

Related Categories:

• micro-RNA Extraction

Protocols

• DNA/RNA Extraction from the Same Material (Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
  A protocol for extraction of both DNA and RNA from the same material typically plant leaves.
  http://www.le.ac.uk/biology/phh4/dnarna.htm
  Added: Fri May 13 2005, Hits: 8363, Reviews: 0  Write review   Cached
• How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
  Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
  Added: Tue May 14 2002, Reviews: 0  Write review  

Trouble Shootings and FAQs

• How to extract RNA from cryopreserved bone marrow cells?
• A single band is observed afterthe isolation of RNA - What is the problem
• RNA isolation - why I always get low 260/280 ratio
• Tips for breaking up tissue in liquid nitrogen for nucleic acid extraction
• RNA Isolation - 8-hydroxyquinoline added to phenol aids in the inactivation of ribonucleases
• Does the degrade of Exracted RNA have a close relationship with this hot weather? Funny but true.
• Tips on DNase treatment for purfcation of RNA
• Cannot amplify>900 bp cDNA from RNA extracted using Trizol
• How to extract total RNA from whole blood for RT-PCR
• No RNA after TRIzol extraction
• RNA Extraction using Trizol - How much Trizol should I use for rat brain tissue?
• RNA extraction (using Trizol) with small cell numbers