Protocols
- Identifying trapped genes by 3' RACE (Megason Lab, Harvard Medical School)
Protocol for Genotyping of FlipTraps by RACE - Nik's hacked RACE protocol
https://wiki.med.harvard.edu/SysBio/Megason/RACEpr...
Added: Thu Feb 02 2012, Hits: 267, Reviews: 0 Write review -
3' RACE (Rapid Amplification of cDNA Ends)
(Langdale Lab, Department of Plant Sciences, University of Oxford)
http://dps.plants.ox.ac.uk/langdalelab/protocols/P...
Added: Wed Feb 11 2009, Hits: 1929, Reviews: 0 Write review -
3' Rapid Amplification of cDNA Ends (RACE)
(Dr. Simon Dawson, Department of Biochemistry University of Nottingham Medical School)
This technique is used to obtain the 3'end of a cDNA, it requires some sequence information internal to the mRNA under study. The sequence information obtained from this technique can be utilised to obtain full length cDNA clones using the 5'RACE technique.
http://www.nottingham.ac.uk/~mbzspd/methods/3RACE_...
Added: Sat Apr 10 2004, Hits: 1521, Reviews: 0 Write review -
5' RACE (Anchor-ligated PCR)
(Langdale Lab, Department of Plant Sciences, University of Oxford)
This protocol uses either oligo d(T) or a gene specific primer (priming from 3’ to 5’) to make cDNA. After RNA hydrolysis, a single stranded adaptor is ligated to the 5’ end of the single stranded cDNA. Amplification is subsequently performed using primers to the adaptor and a gene specific primer (which can be the same as that used in the first step).
http://dps.plants.ox.ac.uk/langdalelab/protocols/P...
Added: Wed Feb 11 2009, Hits: 1881, Reviews: 0 Write review -
5' RACE for EST Production
(Bay Area Functional Genomics Consortium, UCSF )
http://baygenomics.ucsf.edu/protocols/comp1/RACE_g...
Added: Sat Apr 10 2004, Hits: 1593, Reviews: 0 Write review -
Rapid Amplification of mRNA Ends by PCR (RACE)
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 665, Reviews: 0 Write review Cached