Protocols
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In Vitro Transcription, Translation of S35-Radiolabelled Protein and Binding to GST-Fused Proteins o
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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In Vitro Translation
(Promega)
Provides general protocol for coupled single-tube tscription/translation reactions for eukaryotic in vitro translation.
http://www.promega.com/tbs/tm045/TM045.html#i
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Protein Synthesis In Cell Free Systems
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
The process of conversion of a mRNA to a functional protein is known as translation. It involves the attachment of a messenger RNA to the smaller subunit of a ribosome, the addition of the larger subunit, plus initiation by a host of other factors. The entire process can be accomplished in the absence of a cell, if all of the necessary factors are present.
http://homepages.gac.edu/~cellab/chpts/chpt15/ex15...
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