In Vitro Transcription, Translation of S35-Radiolabelled Protein and Binding to GST-Fused Proteins o
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Added: Tue May 14 2002, Hits: 225, Reviews: 0
In Vitro Translation
Provides general protocol for coupled single-tube tscription/translation reactions for eukaryotic in vitro translation.
Added: Tue May 14 2002, Hits: 874, Reviews: 0
Protein Synthesis In Cell Free Systems
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
The process of conversion of a mRNA to a functional protein is known as translation. It involves the attachment of a messenger RNA to the smaller subunit of a ribosome, the addition of the larger subunit, plus initiation by a host of other factors. The entire process can be accomplished in the absence of a cell, if all of the necessary factors are present.
Added: Tue May 14 2002, Hits: 713, Reviews: 0