Protocols
- In-gel digestion protocol
(Goodlett Laboratory, University of Washington)
Includes staining/destaining procedure, reduction and alkylation and protein digestion.
http://goodlett.proteomics.washington.edu/protocol...
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Desalting peptides for Mass Spec.
(Goodlett Laboratory, University of Washington)
http://goodlett.proteomics.washington.edu/protocol...
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In-Gel Digestion Protocol
(UCSF Mass Spectrometry Facility)
http://donatello.ucsf.edu/ingel.html
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In-gel Digestion Protocol for LC-MSn Analysis of an SDSPAGE Separated, Coomassie-Stained Gel
(Proteomics and Biological Mass Spectrometer Facility, Montana State University)
Procedures include excise and wash gel fragments, perform reduction and alkylation of cysteine residues, digest protein sample and sample processing.
http://www.chemistry.montana.edu/facilities/masssp...
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In-Gel Trypsin Digestion Protocol
(Rochester Proteomics Center, University of Rochester Medical Center)
Detailed protocol with a list of recipes.
http://www.urmc.rochester.edu/proteomics/documents...
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In-gel Tryptic Digest for Protein ID by Mass Spectrometry
(Mitchison Lab, Harvard Medical School)
This protocol is based on Shevchenko A, Wilm M, Vorm O, & Mann M. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal Chem 1996, 68:850-8. and has been used with success on both Coomassie and silver-stained gel bands.
http://mitchison.med.harvard.edu/protocols/MSTrypt...
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