Top : Molecular Biology : Protein : Immunoprecipitation (IP) : Co-Immunoprecipitation (Co-IP)

Summary: Co-immunoprecipitation (Co-IP) is a popular technique for the analysis of protein interaction. The procedure includes: 1) An antibody specific to the protein of interest is added to a cell lysate. 2) The antibody-protein complex is then precipitated usually using protein-G or protein-A sepharose which binds most antibodies. If there are any protein/molecules that bind to the first protein, they will also be precipitated. 3) Co-precipitated protein can then be identified by Western blot analysis or by sequencing a purified protein band.