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Top : Molecular Biology : Protein : Immunoprecipitation (IP) : Chromatin Immunoprecipitation (ChIP Assay) : N-ChIP
Overview: N-ChIP, different from X-ChIP which uses chromatin fixed with formaldehyde and fragmented by sonication, uses native chromatin prepared by nuclease digestion of cell nuclei.
Protocols
  • ChIP on Native Chromatin from Cells and Tissues (The Epigenome Network of Excellence )
    This protocol describes a ChIP assay performed on native chromatin extracted from cells, or tissues, to analyse histone methylation and acetylation at specific chromosomal sites. In addition, it describes different PCR-based methods that allow the analysis of a locus of interest in chromatin precipitated with antibodies to specific histone marks.
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Sat Mar 18 2006, Hits: 2378, Reviews: 0  Write review   Cached
  • Chromatin Preparation from Cultured Cells (Chromatin and Gene Expression Group, The University of Birmingham, UK)
    Detailed protocol with receipes.
    http://medweb.bham.ac.uk/research/chromatin/protoc...
    Added: Sun Jan 08 2006, Hits: 1338, Reviews: 0  Write review   Cached
  • Immunoprecipitation of Acetylated H4 from Unfixed Chromatin (Chromatin and Gene Expression Group, The University of Birmingham, UK)
    http://medweb.bham.ac.uk/research/chromatin/protoc...
    Added: Sun Jan 08 2006, Hits: 452, Reviews: 0  Write review   Cached
  • N-ChIP and X-ChIP protocols (Abcam)
    Expert protocols and tips for N-ChIP and X-ChIP.
    http://www.abcam.com/index.html?pageconfig=resourc...
    Added: Fri Jan 25 2008, Hits: 350, Reviews: 0  Write review   Cached
  • Native ChIP (N-ChIP) Protocol PDF file (Abcam)
    Step-by-step procedure including preparation of native chromatin from cultured human cells, micrococcal nuclease digestion, immunoprecipitation, etc.
    http://www.abcam.com/ps/pdf/protocols/N-ChIP.pdf
    Added: Sun Jan 08 2006, Hits: 1476, Reviews: 0  Write review