Protocols
- Protein Assay (David R. Caprette, Rice University )
A list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/amount of sample needed, subjective comments on accuracy and convenience, and major interfering agents. Procedural details, equipment requirements, and references are outlined in the individual assay documents. Two categories of methods are included: Absorbance assays and colorimetric assays (Modified Lowry, Biuret, Bradford assay and Bicinchoninic Acid (Smith)).
http://www.ruf.rice.edu/~bioslabs/methods/protein/...
Added: Sun Sep 29 2002, Hits: 3825, Reviews: 0 Write review Cached - Silver Staining of Proteins in Gel (Contributed by Sharad Purohit)
Added: Tue May 14 2002, Reviews: 0 Write review -
Alkaline Phosphatase in Situ Assay
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
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Coomasie Blue Staining of Protein Gel
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Coomasie Brilliant Blue R 250 is the most commonly used staining procedure for the detection of proteins. It is the method of choice if SDS is used in the electrophoresis of proteins, and is sensitive for a range of 0.5 to 20 micrograms of protein.
http://homepages.gac.edu/~cellab/chpts/chpt4/ex4-3...
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Derivation of Binding Constants by Inhibition Equilibrium Dialysis
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/Equilibrium_...
Added: Wed Aug 14 2002, Hits: 747, Reviews: 0 Write review -
Derivation of Binding Constants by Scatchard Analysis
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/Scatachard_A...
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Detection of Actin Polymerization with Pyrene Actin
(Yu-li Wang's Lab, University of Massachusetts Medical School )
http://ylwang.umassmed.edu/protocol/pa/pyaassay.ht...
Added: Fri Oct 17 2003, Hits: 218, Reviews: 0 Write review Cached - Detection of Glycoproteins on Blot (Contributed by Sharad Purohit, Paller's Lab)
Added: Tue May 14 2002, Reviews: 0 Write review -
HLA-B27 Binding Assay
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/B27_binding_...
Added: Wed Aug 14 2002, Hits: 665, Reviews: 0 Write review -
Immunolocalization of photosynthetic proteins
(Sinha Lab, University of California, Davis)
The protocol works well with abundant photosynthetic cytoplasmic or chloroplast localized proteins. It should work for other abundant proteins too. For detecting rare proteins, nuclear localized proteins and possibly cell wall proteins, modifications may be needed.
http://www-plb.ucdavis.edu/labs/sinha/immuno.pdf
Added: Wed Sep 04 2002, Hits: 816, Reviews: 0 Write review -
Phosphoamino Acid Analysis of non-TCA Precipitable Proteins
(Woodgett Lab)
http://kinase.uhnres.utoronto.ca/methods/PAA.html
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Protein detection
(Aberdeen's Lab)
The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.
http://www.abdn.ac.uk/~mmb023/protocol.htm#protein...
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Protein detection(Silver staining)
(ExPASy)
The application of the 2-D PAGE technology to separate, analyze and characterize proteins contained in biological samples would not have been possible without the development of complementary detection methods. Perhaps today one of the most popular non-radioactive protein detection is the silver staining which is 100-fold more sensitive than Coomassie Brilliant Blue staining
http://au.expasy.org/ch2d/protocols/protocols.fm4....
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Protein Limited Proteolysis
(Chazin Lab, Center for Structural Biology, Vanderbilt University)
This protocol describes the general procedure for setting up a limited proteolysis reaction. An experiment like this is useful for determining the sizes, timed order of appearance, and relative amounts of fragments produced from the protein of interest by digestion with a protease. From the results, you may be able to infer the presence of stable subdomains. It is also possible to obtain evidence of binding of one protein to another by running two identical sets of proteolysis experiments, one with the putative target, and one without.
http://structbio.vanderbilt.edu/chazin/wisdom/labp...
Added: Sat Oct 18 2003, Hits: 1170, Reviews: 0 Write review Cached -
Protein Staining
(Molecular Profiling Initiative, NCI)
Procedures includes Coomassie blue staining, silver nitrate staining and zinc imidazole negative staining
http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAn...
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Silver Stain for SDS PAGE
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://labs.fhcrc.org/hahn/Methods/biochem_meth/si...
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Silver Staining of Acrylamide Gels
(Waters Lab)
http://www.molbio.princeton.edu/labs/waters/silver...
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Silver Staining of Protein Gels
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt4/ex4-4...
Added: Tue May 14 2002, Hits: 832, Reviews: 0 Write review Cached