Protocols
- Coomassie and Silver Staining of Polyacrylamide Gels ( Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
Two methods are described with troubleshooting and recipes.
http://omrf.ouhsc.edu/~frank/SILVER.html
Added: Thu Sep 01 2005, Hits: 2635, Reviews: 0 Write review Cached - Lowry Protein Assay (Yu-li Wang's Lab, University of Massachusetts Medical School )
Detailed procedures with materials.
http://ylwang.umassmed.edu/protocol/pa/lowry.htm
Added: Fri Oct 17 2003, Hits: 9559, Reviews: 0 Write review Cached - Protein Assay (David R. Caprette, Rice University )
A list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/amount of sample needed, subjective comments on accuracy and convenience, and major interfering agents. Procedural details, equipment requirements, and references are outlined in the individual assay documents. Two categories of methods are included: Absorbance assays and colorimetric assays (Modified Lowry, Biuret, Bradford assay and Bicinchoninic Acid (Smith)).
http://www.ruf.rice.edu/~bioslabs/methods/protein/...
Added: Sun Sep 29 2002, Hits: 6672, Reviews: 0 Write review Cached - Silver Staining of Proteins in Gel (Contributed by Sharad Purohit)
Added: Tue May 14 2002, Reviews: 0 Write review -
Alkaline Phosphatase in Situ Assay
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Sat Nov 02 2002, Hits: 690, Reviews: 0 Write review Cached -
BCA (Bicinchoninic Acid) Protein Assay
(Department of Biology, University of Southern Colorado )
Detailed procedures and reaction illustration.
http://csm.colostate-pueblo.edu/biology/dcaprio/41...
Added: Sun Sep 29 2002, Hits: 4630, Reviews: 0 Write review Cached -
BCA (Bicinchoninic Acid) Protein Assay
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
BCA, in the form of its water-soluble salt, is a sensitive, stable and highly specific reagent for the cuprous ion (Cu+). Peptide bonds and four amino acids (cysteine, cystine, tryptophan, and tyrosine) have been reported to be responsible for color formation in protein samples when assayed with BCA.
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Sun Sep 29 2002, Hits: 5128, Reviews: 0 Write review Cached -
BCA Protein Assay
(Yu-li Wang's Lab, University of Massachusetts Medical School )
http://ylwang.umassmed.edu/protocol/pa/bca.htm
Added: Fri Oct 17 2003, Hits: 4017, Reviews: 0 Write review Cached -
Coomasie Blue Staining of Protein Gel
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Coomasie Brilliant Blue R 250 is the most commonly used staining procedure for the detection of proteins. It is the method of choice if SDS is used in the electrophoresis of proteins, and is sensitive for a range of 0.5 to 20 micrograms of protein.
http://homepages.gac.edu/~cellab/chpts/chpt4/ex4-3...
Added: Tue May 14 2002, Hits: 1846, Reviews: 0 Write review Cached -
Derivation of Binding Constants by Inhibition Equilibrium Dialysis
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/Equilibrium_...
Added: Wed Aug 14 2002, Hits: 461, Reviews: 0 Write review