Protocols
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Immunoprecipitation and Immune Complex kinase assay
(Sefton Lab, Salk Institute)
http://pingu.salk.edu/~sefton/Hyper_protocols/ipPr...
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Non-TCA Phosphoamino Acid Determinations
(Woodgett Lab)
http://kinase.uhnres.utoronto.ca/methods/PAA.html
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Phosphoamino Acid Analysis
(Sefton Lab)
http://pingu.salk.edu/~sefton/Hyper_protocols/Paa....
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Protein assays
(Lamond Lab, University of Dundee)
Two protein assay protocols can be found on this page: - Splicing assays
- Phosphatase assays
http://www.lamondlab.com/LTprotAssays.htm
Added: Thu May 08 2003, Hits: 343, Reviews: 0 Write review Cached -
Protein Kinase A Assay
(Murphy Lab, Department of Pharmacology, Emory University)
http://www.pharm.emory.edu/tmurphy/Methods/PKA_Ass...
Added: Tue Mar 03 2009, Hits: 1215, Reviews: 0 Write review -
Protein Kinase Assay after Denaturation and Renaturation
(BD PharMingen)
This protocol can be used for general purposes.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 846, Reviews: 0 Write review Cached -
Protein Kinase Assay with an Immunocomplex
(BD PharMingen)
Detailed procedures including cell lysate preparation, immunoprecipitation of the protein and kinase assay. The protocol is for general use.
http://www.bdbiosciences.com/pharmingen/protocols/...
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SAPK/Jun Kinase Assays
(Woodgett Lab)
Detailed protocol with recipes
http://kinase.uhnres.utoronto.ca/methods/SAPKassay...
Added: Tue May 14 2002, Hits: 666, Reviews: 0 Write review Cached -
Tyrosine Kinase Assay Using Synthetic Peptides
(Todd Miller, State University of New York at Stony Brook)
Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized cells or for enzyme kinetic studies. This is a detailed protocol for tyrosine kinase assay.
http://pkr.sdsc.edu/html/pk_protocols/tyr_synpep_a...
Added: Tue May 14 2002, Hits: 313, Reviews: 0 Write review Cached