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Protocols
- Competitive RT-PCR (Dieter Kaufmann)
For quantifying mRNA, internal standard RNAs are added in a defined quantity to the RNA sample prior to the RT reaction. The resulting standard cDNA is coamplified with the same primers as the endogenous target sequence. Its PCR product is approximately 50 nucleotides smaller. This method allows measurement of small differences (as low as factor 2) in mRNA amount between RNA samples.
http://www.uni-ulm.de/~dkaufman/rt-pcr.html
Added: Tue May 14 2002, Hits: 6988, Reviews: 0 Write review Cached - Quantitative RT-PCR
(The Morimoto Lab, Northwestern University)
This 9-page protocol describes every aspects of quantitative RT-PCR in great detail, including the principles, RNA preparation, primer design, preparation of internal control...
http://www.biochem.northwestern.edu/ibis/morimoto/...
Added: Sun Mar 28 2004, Hits: 15989, Reviews: 0 Write review - Quantitative RT-PCR and Other PCR Procedures (Jack Vanden Heuvel, Penn State University)
A must-read protocol for PCR beginners as well as verterans. It touches in depth aspects specific to quantitative RT-PCR as well as those common to standard PCR.
http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvh...
Added: Sun Mar 28 2004, Hits: 15033, Reviews: 0 Write review -
Competitive Quantitative RT-PCR
(Ambion)
Detailed protocol for competitive quantitative RT-PCR
http://www.ambion.com/techlib/prot/bp_1760.pdf
Added: Tue May 14 2002, Hits: 4297, Reviews: 0 Write review -
Semi-Quantitative RT-PCR
(Mike A. Dyer)
From RNA isolation, reverse transcription to PCR...
http://axon.med.harvard.edu/~cepko/protocol/mike/R...
Added: Tue May 14 2002, Hits: 7286, Reviews: 0 Write review Cached -
Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa
(Biological Procedures Online )
a semiquantitative RT-PCR protocol optimized to extract RNA from as little as 10,000 cells and to measure the expression levels of several target mRNAs from each sample
http://www.biologicalprocedures.com/bpo/arts/1/20/...
Added: Wed Sep 25 2002, Hits: 7231, Reviews: 3 Read reviews Write review Cached