Protocols
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Degenerate PCR
(Michael Koelle)
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
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Degenerate PCR
(Michael Koelle, at another server)
Detailed guide in degenerate PCR, including primer design, reaction conditioning and product analysis.
http://info.med.yale.edu/mbb/koelle/protocols/prot...
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Degenerate PCR Primer Design
(Molecular Biology Techniques Manual)
For amplification of cognate sequences from different organisms, or for "evolutionary PCR", one may increase the chances of getting product by designing "degenerate" primers: these would in fact be a set of primers which have a number of options at several positions in the sequence so as to allow annealing to and amplification of a variety of related sequences.
http://www.mcb.uct.ac.za//pcroptim.htm#Degenerate
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Degenerate PCR-A Short Guide
(Atle M. Bones)
http://boneslab.bio.ntnu.no/degpcrshortguide.htm
Added: Tue May 14 2002, Hits: 4969, Reviews: 0 Write review Cached