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Protocols
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Digoxigenin-UTP RNA Labeling
(Frank Bottone Jr.)
http://www.unc.edu/~fbottone/protocol/dig_rna.html
Added: Tue May 14 2002, Hits: 966, Reviews: 0 Write review Cached -
E.coli Total RNA Labeling Protocol for Spotted Microarray
(University of Wisconsin E. coli Genome Project)
Describes RNA preparation, labeling and cleaning up.
http://www.genome.wisc.edu/resources/protocols/rna...
Added: Mon Sep 16 2002, Hits: 464, Reviews: 0 Write review Cached -
Reverse Transcription and aa-UTP Labeling of RNA
(DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
Protocol for making cDNA with aa-dUTP incorporation and labeling cDNA via chemical coupling of fluorescent dyes.
http://www.microarrays.org/pdfs/aaUTP_RT_Coupling....
Added: Tue May 14 2002, Hits: 487, Reviews: 0 Write review -
Reverse Transcription and Labeling of Human RNA
(DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
Protocol for direct incorporation of fluorescent nucleotides into cDNA reverse transcribed from human mRNA.
http://www.microarrays.org/pdfs/HumanRNALabel.pdf
Added: Tue May 14 2002, Hits: 464, Reviews: 0 Write review -
RNA (Riboprobe) Labeling
(Laboratory of Cellular and Molecular Regulation)
Typically, the plasmid containing the cDNA to be transcribed is linearized with a restriction enzyme. It may be useful to restrict a large amount (about 10µg) of plasmid if that particular probe is to be repeatedly made. Another approach is to generate PCR fragments of the desired cDNA using primers that contain different RNA polymerase transcription initiation sequences. The PCR fragments are gel-purified and used below instead of the linearized plasmid.
http://intramural.nimh.nih.gov/lcmr/snge/Protocols...
Added: Sun Jun 05 2005, Hits: 773, Reviews: 0 Write review Cached